Characterization of fructans in fermentation products with GPC/SEC

Recent efforts to improve the efficiency of biorefineries aim to take advantage of otherwise discarded side products. In this application, a sample of biotechnological origin from cup plant (Silphium perfoliatum) was characterized in regards of the distribution of fructan polymers and other sugar contents. This was achieved using size exclusion chromatography (SEC) in combination with refractive index detection (RID). Next, the method was scaled up to a semi-preparative dimension in order to separate oligomers from monomers.

Methode	HPLC
Modus	GPC/SEC
Substanzen	Fructan polymers
Schlüsselwörter	Biotechnology, purification, size exclusion chromatography, refractive index detection, cup plant stems, fructans, oligomers, polymers, monomers, gel permeation chromatography
Version	Version 1 / 03/2021