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Boosting your sensitivity - Analysis of fluorescence-labeled proteins with semi-preparative FPLC and FLD

Applikationsnr.: VTN0006

Boosting your sensitivity - Analysis of fluorescence-labeled proteins with semi-preparative FPLC and FLD

In many bioscience applications, fluorescence-labeled proteins are used to analyze
cellular and biological functions. Here, fluorescein (FITC) and bovine serum albumin
(BSA) were used as model molecules. We tested the integration of a fluorescence
detector (FLD) into a semi-preparative FPLC system and evaluated the analysis of FITC
labeled BSA in comparison with classical UV/VIS detection. The use of a fluorescence
detector increases the sensitivity of the chromatographic analysis significantly.

  • Overlay of FITC-BSA peak measured with fluorescence (red) and UV 280 nm (blue). Sample 1 with 0.8 μg

  • Overlay of FITC-BSA peak measured with fluorescence (red) and UV 280 nm (blue). Sample 2 with 1.3 μg

  • FPLC System

Technische Daten

Spezifikationen

Methode FPLC
Modus SEC
Substanzen Fluorescein isothiocyanate isomer I (FITC), Bovine Serum Albumin (BSA)
Schlüsselwörter SEC, FPLC, AZURA FPLC, fluorescence, BSA, FITC, fluorescein-labeled protein
CAS Nummer 3326-32-7, 9048-46-8
Version 001/2020-06-18

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