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Streamlining of equipment: Hybrid system for preparative and analytical LC

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Application No.: VTN0010

Streamlining of equipment: Hybrid system for preparative and analytical LC

 

When separating and purifying molecules by liquid chromatography (LC), it is necessary to develop the method with a preparative high-performance liquid chromatography (HPLC) system. The purification process is often verified for quality and quantity of the products by analytical HPLC. In this case, two LC systems, a preparative and an analytical, are needed for a purification process and the following analysis.

In this technical note a combination of these two systems is shown. It describes in detail the system set up and the different method steps. The system is capable of isolating a single substance from a mixture and afterwards performing an automated purity analysis. For proof of concept, caffeine was purified from paracetamol and analysed within the same system.

Technical Data

Application details

Method Preparative and analytical HPLC
Mode RP
Substances Paracetamol, caffeine
Key words Hybrid system, preparative hplc, analytical hplc, purification, purify and analyze, caffeine, paracetamol
CAS number Paracetamol: CAS 103-90-2; Caffeine: CAS 58-08-2
Version 1 / 10-2020

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