When developing an HPLC method, one of the initial steps involves screening a variety of columns to determine which offers the most effective separation of the analytes. While this can be performed manually, an automated approach is generally preferable, as it enables rapid switching between multiple columns within a short timeframe. Automated valve systems present a viable solution for this purpose. Two common configurations are employed: (1) using two multiposition valves with columns arranged between them, or (2) employing a specialized column switching valve designed to accommodate all columns simultaneously. Here, we introduce a new high-pressure column switching valve capable of connecting up to four columns, in addition to providing a bypass path. To demonstrate its utility, the separation profiles of a standard mixture were evaluated on four different columns under two distinct solvent gradient conditions. This exemplary screening illustrates the advantages of employing a single column switching valve for method development and other applications that require efficient and reliable switching between multiple columns.
Scheme system components for column selection. A) classic set-up with two 6 port multiposition valves B) optimized set-up with high pressure column selection valve
Set-up analytical HPLC system for column selection with flow scheme. A) classic set-up with two 6 port multiposition valves B) optimized set-up with high pressure column selection valve
Connection ports of KNAUER column selection valve; 1) flow passes through column 1; 2) flow passes through column 2
Overlay chromatograms with water/methanol; 1) vanillic acid, 2) syringic acid, 3) p-coumaric acid, 4) ferulic acid, 5) benzoic acid, 6) quercetin, 7) kaempferol; 4 μl injection, 0.6 ml/min, 25°C
Overlay chromatograms with water/acetonitrile; 1) vanillic acid, 2) syringic acid, 3) p-coumaric acid, 4) ferulic acid, 5) benzoic acid, 6) quercetin, 7) kaempferol; 4 μl injection, 0.6 ml/min, 25°C
| Method | HPLC |
|---|---|
| Mode | RP |
| Substances | Vanillic acid, syringic acid, p-coumaric acid, ferulic acid, benzoic acid, quercetin, kaempferol |
| Key words | Column selection valve, method development, solvent screening, stationary phase screening, automatization |
| CAS number | 121-34-6, 530-57-4, 501-98-4, 1135-24-6, 65-85-0, 117-39-5, 520-18-3 |
| Version | Version1 / 12/2024 |
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