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Streamlining of equipment: Hybrid system for preparative and analytical LC

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Applikationsnr.: VTN0010

Streamlining of equipment: Hybrid system for preparative and analytical LC

 

When separating and purifying molecules by liquid chromatography (LC), it is necessary to develop the method with a preparative high-performance liquid chromatography (HPLC) system. The purification process is often verified for quality and quantity of the products by analytical HPLC. In this case, two LC systems, a preparative and an analytical, are needed for a purification process and the following analysis.

In this technical note a combination of these two systems is shown. It describes in detail the system set up and the different method steps. The system is capable of isolating a single substance from a mixture and afterwards performing an automated purity analysis. For proof of concept, caffeine was purified from paracetamol and analysed within the same system.

Technische Daten

Spezifikationen

Methode Preparative and analytical HPLC
Modus RP
Substanzen Paracetamol, caffeine
Schlüsselwörter Hybrid system, preparative hplc, analytical hplc, purification, purify and analyze, caffeine, paracetamol
CAS Nummer Paracetamol: CAS 103-90-2; Caffeine: CAS 58-08-2
Version 1 / 10-2020

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