This application compares the automated purification of antibodies with the AZURA Bio-Two Step Purification System with different columns. Capacity and yield of the purified proteins were compared and revealed no significant differences in the performances of the two investigated column sets.
Overlay of chromatograms of the two step antibody purification
SDS-PAGE at different purification steps
| Method | FPLC |
|---|
| Mode | Affinity |
|---|---|
| Substances | human Plasma |
| Key words | FPLC, Two Step Purification, Plasma, antibodies, SEC, Affinity, automation, Sepapure Protein A, Sepapure Desalting |
| CAS number | - |
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