Neonicotinoids are active substances used in plant protection products to control harmful insects. They are systemic pesticides, which means that they are taken up by the plant and transported through its leaves, flowers, roots, and stems, as well as pollen and nectar. Neonicotinoids affect the central nerve system of insects, leading to eventual paralysis and death [1]. Three honey samples from different sources were analysed for neonicotinoid content according to current regulating guidelines via a fast and simple HPLC method.
Chromatogram of mixed standard at 10 μg/mL, 1) thiamethoxam, 2) clothianidin, 3) imidacloprid, 4) acetamiprid
Overlay of standard at 2.50 μg/mL (blue) and cleaned sample of fruit blossom honey (red), Zoom in
Overlay of standard at 2.50 μg/mL (blue) and cleaned sample of KNAUER honey (red)
Overlay of standard at 2.50 μg/mL (blue) and cleaned sample of canola honey (red)
| Method | HPLC |
|---|---|
| Mode | RP |
| Substances | Thiamethoxam, clothianidin, imidacloprid, acetamiprid |
| Key words | Insecticides, pesticides, neonicotinoids, honey, QuEChERS, neuro-active insecticides, bee losses, dispersive SPE |
| CAS number | 153719-23-4, 210880-92-5, 138261-41-3, 135410-20-7 |
AZURA® Pump P 6.1L (HPG), with 10 ml pump head (stainless steel), degasser and mixer (400 µl)
Article No.: APH35EA
Show product
AZURA® Autosampler AS 6.1L analytical HPLC autosampler, 700 bar
Article No.: AAA00AA
Show product
AZURA® Detector DAD 6.1L Diode array detector DAD 6.1L without flow cell 190 - 1000 nm, incl. test cell
Article No.: ADC11
Show product
50 mm path length, 6 µl, 1/16", 50 bar, High Sensitivity LightGuide Flow cell cartridge for AZURA® Detector DAD/MWD
Article No.: AMD59XA
Show product
Column thermostat AZURA CT 2.1 for constant temperatures and reproducible results
Article No.: ATC00
Show product
Article No.: 25EE182E2J
Show product
ClarityChrom® single instrument license for one time base
Article No.: A1670
Show product
ClarityChrom® option for PDA data processing
Article No.: A1676
Show productWe are happy to help you