A latest approach in bioethanol generation is the usage of yeast and bacteria that uses C5 sugars for fermentation and the valorization of bio refinery by products. Here it is shown that a hemicellulose-like fermentation mash has a high content of the artificial sweetener xylitol and that its purification by HPLC can be accomplished using polymer based Eurokat columns in high recovery (95%) with a high purity (99%). The refractive index detector's Extended Dynamic Range (EDR) feature was used for preparative experiments.
Sample analysis: 1. Xylose; 2. Arabinose; 3. Glycerol; 4. Mannitol; 5. Xylitol
Batch purification: Xylitol (yellow)
Fraction analysis
| Method | HPLC |
|---|
| Mode | Ion exclusion, Ligand exchange |
|---|---|
| Substances | xylose (D +), arabinose (D -), glycerol (D -), mannitol, xylitol |
| Key words | Xylose, arabinose, glycerol, mannitol, xylitol, fermentation broth, carbohydrates, sugar alcohols, HPLC purification, SEC–IEX |
| CAS number | 58-86-6; 10323-20-3; 56-81-5; 69-65-8; 87-99-0 |
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