Complete solutions for FPLC (Fast Protein Liquid Chromatography) on a small footprint: AZURA® FPLC systems combine flexibility and reliability. The AZURA® FPLC is the perfect choice for your protein purification task.
Design your AZURA Bio purification system to your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection as well as fraction collection enable the user to automate the purification process.
A range of different detectors let you keep track of your target molecules. A wide flow rate range and compatibility with columns from all vendors offer maximum flexibility. The intuitive software PurityChrom® combines all the advantages of a versatile purification software.
From simple to complex, from Lab to Pilot scale: Design your AZURA® FPLC system according to your purification task!
Because of their modularity, AZURA® Bio Lab devices can be flexibly arranged with each other to a bio purification system that matches your requirements. Accordingly, AZURA® offers a solution even for complex purification tasks like for example a 3-step purification process including the steps: 1. Simultaneous capturestep of up to 5 samples to shorten the time for sample loading 2. Sequential intermediate or desalting step via a second column 3. Sequential polishing or desalting step via a third column inlcuding fraction collection
All of these steps can be perfectly managed by the FPLC software PurityChrom 6 including data signal triggered fractionation.
AZURA® Bio Lab allows you to create FPLC systems with highest independence. Just pick your modules and create the system yourself.
You choose the method-
We have the Bio purification system for you
Size Exclusion (SEC)
Affinity (AC)
Ion-Exchange (IEX)
Hydrophobic Interaction (HIC)
All in One
Size Exclusion Chromatography (SEC)
SEC, also referred to as gel permeation chromatography (GPC), separates the components by molecular size while the sample is passing through the stationary phase (one step).
The deciding factor is the pore size distribution of the stationary phase. However, it is not a sieving effect, but a diffusion effect, because large molecules are eluted faster than the small ones.
The smaller molecules can better penetrate into the pores of the stationary phase and thus stay longer in the column than large molecules, which have less space for movement and therefore are more strongly entrained with the eluent. Therefore, the big molecules elute first.
Affinity chromatography is a multi-step process (binding, washing, elution) characterized by specific binding of the target molecules to the column material. Therefore, the ligand of the stationary phase must be matched to the target molecules. In a washing step, all non-specifically bound portions of the sample are removed. The subsequent elution step releases the target molecules.
Ion exchange chromatography separates molecules based on their total charge.
It is a competitive process in which target molecules and all molecules in the sample featuring a polarity of the same direction are first "nonspecifically" bound to the stationary phase and then gradually eluted (2 steps).
For example, a cation exchanger is used for target molecules with a positive overall charge.
The stationary phase itself is of opposite charge, that is negatively charged. Initial binding to the stationary phase occurs under low ionic strength conditions, i.e. the mobile phase with the sample has a low electrolyte content. The elution is achieved by a salt gradient. By increasing the salt concentration, proteins with a weak charge elute first, while at higher salt concentrations proteins with a strong charge elute later.
In protein purification processes different methods are usually combined in a more complex purification strategy. Multi-Method systems can be used to perform all methods used in your purification strategy on one system.
Choosing between a multitude of buffers and samples is essential when auomating a purification routine. To do so you can choose to add multipleinlet valves to the system pump and sample pump to widen your selection.
Up to three valves can be combined in an FPLC module docking station. A combination between sample pump, small detector and valves in one docking station is also possible.
Within an FPLC purification differing sample sizes are common, ranging from microliter to liter. To handle the changing sample sizes one often switches between a sample pump and sample loops for sample injection. A multi-injection valve is perfect to do so in a lab system.
FPLC purifications are usually a multi step process. And each step relies on a specific column. To be able to switch between these columns fast and easy the column selection valve can be used in a lab system.
An outlet valve module can be used as a standalone fractionation valve or in combination with a fraction collector. Besides fraction collection the outlet valve can also be used to transform your standard Lab system into a two step purification system.
Up to three valves can be combined in an FPLC module docking station. A combination between sample pump, small detector and valves in one docking tation is also possible.
Discover the our FPLC columns based on Agarose and Dextran. KNAUER offers prepacked FPLC columns and resins for all common FPLC applications: Size Exclusion Chromatography (SEC), Affinity Chromatography (AC) and Ion-Exchange Chromatography (IEX)
The PurityChrom® is a powerful software to control your FPLC system. Get familiar with PurityChrom® in shortest time and with no effort due to the intuitive and clearly structured user interface. Choose a time- or volume based workflow by just clicking one button. Create methods with highest flexibility to realize complex application without losing easy handling. Offline licenses for creating methods and data evaluation are for free.
Sometimes it is the little things that produce the big results. In addition to systems and devices, KNAUER offers a wide range of Accessories and Supplies. These parts are compatible with all commonly used HPLC or FPLC systems, columns, pumps, as well as detectors and were designed to simplify everyday laboratory life.
Accessory
Features
Benefit
Pressure
Control
• Contains two pressure sensors
• Automatic determination of pressure difference with PurityChrom® software
• Connect 1/16” or 1/8” tubings
• Up to 250 ml/min and 60 bar
Monitor pressure over the column bed and protect column from damage
Air Sensor
• Detect end of buffer or end of sample with PurityChrom® software
• Up to four or twelve air sensors per system via gameport adapter or event box
• For transparent tubings with 1/16” or 1/8” or 1/4” outer diameter
Protect column from air damage and support automation ( e.g. sample injection)
AZURA
Click
• Attach air sensor, pressure control, AZURA Organizer or your interface box to the side panel of your AZURA L device
Organize your system
AZURA
Organizer
• Attach columns from 5 mm to 26 mm diameter, falcon tubes, a back pressure regulator or a pH flow cell
Organize accessories directly at the system and reduce dead volume
Back pressure regulator (BPR)
• Apply a constant back pressure to your system
• Freely adjustable between 1-20 bar or 20-103 bar
Prevent formation of air bubbles after the column which disturb detector signal
The complete brochure gives you an excellent overview of protein purification with the flexible AZURA Bio FPLC systems. Get to know the components of these FPLC systems and the different possibilities in detail.
We have designed our systems to meet your purification challenges!
